Figure 8. MSX1 directly binds to the ICAM1 and VCAM1 promoter. (A and B) Epigenome profiling of the ICAM1 (A) and VCAM1 (B) promoter representing DNase-seq and ChIP-seq against H3K27ac, H3K4m1, H3K4m3, and H3K27m3 data tracks from the University of California, Santa Cruz Genome Browser of the ICAM1 and VCAM1 promoter in HUVECs. Dotted lines show regions of interest, and J indicates predicted MSX1 binding sites from the JASPAR TF binding profile database. (C–F) qRT-PCR analysis for ICAM1 (site 1), VCAM1 (site 2), and two negative control genomic regions upon FLAG- or mouse IgG–based ChIP in HUAECs transduced with Cherry or FLAG-MSX1 plasmid. Data are represented as efficiency of the ChIP assay. Error bars represent the SEM. n = 6. *, P < 0.05 versus the indicated condition.
Figure 8.

MSX1 directly binds to the ICAM1 and VCAM1 promoter. (A and B) Epigenome profiling of the ICAM1 (A) and VCAM1 (B) promoter representing DNase-seq and ChIP-seq against H3K27ac, H3K4m1, H3K4m3, and H3K27m3 data tracks from the University of California, Santa Cruz Genome Browser of the ICAM1 and VCAM1 promoter in HUVECs. Dotted lines show regions of interest, and J indicates predicted MSX1 binding sites from the JASPAR TF binding profile database. (C–F) qRT-PCR analysis for ICAM1 (site 1), VCAM1 (site 2), and two negative control genomic regions upon FLAG- or mouse IgG–based ChIP in HUAECs transduced with Cherry or FLAG-MSX1 plasmid. Data are represented as efficiency of the ChIP assay. Error bars represent the SEM. n = 6. *, P < 0.05 versus the indicated condition.

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