Figure 6. MSX1 activates the collateral endothelium to a proadhesive state for leukocyte infiltration. (A) Staining for CD45 to mark leukocytes (red), αSMA (green), and DAPI (blue) in collateral arteries of tamoxifen-treated Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl mice 3 d after surgery. (B and C) Quantification of leukocyte infiltration in the perivascular region of collateral arteries in Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl (B) and Sm22-Cre;Msx1fl/fl:Msx2fl/fl mice (C) 3 d after surgery. Data represent the number of CD45-positive cells per vessel. n = 4 Cdh5-CreERT2-;Msx1fl/fl:Msx2fl/fl; n = 5 Cdh5-CreERT2+;Msx1fl/fl:Msx2fl/fl; n = 4 Sm22-Cre−;Msx1fl/fl:Msx2fl/fl; and n = 4 Sm22-Cre+;Msx1fl/fl:Msx2fl/fl mice. *, P < 0.05 versus the indicated condition. (D and E) Representative stainings for ICAM1 (D) and VCAM1 (E) in green, αSMA (red), and DAPI (blue) in the adductor of tamoxifen-treated Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl mice 3 d after ligation. (F) Corresponding quantification of the fluorescence intensity for ICAM1 and VCAM1 in the intima of collateral arteries. Data are represented as fluorescence intensity per intimal area (square micrometers). n = 4 Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl and n = 5 Cdh5-CreERT2+;Msx1fl/fl:Msx2fl/fl mice. *, P < 0.05. Error bars represent the SEM. Bars, 10 µm.
Figure 6.

MSX1 activates the collateral endothelium to a proadhesive state for leukocyte infiltration. (A) Staining for CD45 to mark leukocytes (red), αSMA (green), and DAPI (blue) in collateral arteries of tamoxifen-treated Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl mice 3 d after surgery. (B and C) Quantification of leukocyte infiltration in the perivascular region of collateral arteries in Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl (B) and Sm22-Cre;Msx1fl/fl:Msx2fl/fl mice (C) 3 d after surgery. Data represent the number of CD45-positive cells per vessel. n = 4 Cdh5-CreERT2-;Msx1fl/fl:Msx2fl/fl; n = 5 Cdh5-CreERT2+;Msx1fl/fl:Msx2fl/fl; n = 4 Sm22-Cre−;Msx1fl/fl:Msx2fl/fl; and n = 4 Sm22-Cre+;Msx1fl/fl:Msx2fl/fl mice. *, P < 0.05 versus the indicated condition. (D and E) Representative stainings for ICAM1 (D) and VCAM1 (E) in green, αSMA (red), and DAPI (blue) in the adductor of tamoxifen-treated Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl mice 3 d after ligation. (F) Corresponding quantification of the fluorescence intensity for ICAM1 and VCAM1 in the intima of collateral arteries. Data are represented as fluorescence intensity per intimal area (square micrometers). n = 4 Cdh5-CreERT2;Msx1fl/fl:Msx2fl/fl and n = 5 Cdh5-CreERT2+;Msx1fl/fl:Msx2fl/fl mice. *, P < 0.05. Error bars represent the SEM. Bars, 10 µm.

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