Figure 2. Endothelial MSX1 expression is induced by a changing hemodynamic environment. (A) Expression analysis by qRT-PCR of HUAECs seeded on fibronectin-coated chambers kept in static conditions or exposed to different LSS levels over time. MSX1 mRNA expression is represented relative to the static condition. n = 3 for 5 dynes and n = 8 for 12 and 20 dynes. Error bars represent the SEM. *, P < 0.05 versus static condition. (B) Western blot of total cell lysates of HUAECs kept in static conditions or exposed to LSS of 20 dynes/cm2 for 1 h for MSX1, pSMAD1/5/8, SMAD1, and GAPDH. (C) IF staining on HUAECs with and without exposure to LSS of 20 dynes/cm2 for 1 h for MSX1 (red), phosphorylated (p)SMAD1/5/8 (green), and Hoechst (nuclear marker in blue) and the corresponding brightfield pictures. The arrows represent the flow direction. Bars, 50 µm.
Figure 2.

Endothelial MSX1 expression is induced by a changing hemodynamic environment. (A) Expression analysis by qRT-PCR of HUAECs seeded on fibronectin-coated chambers kept in static conditions or exposed to different LSS levels over time. MSX1 mRNA expression is represented relative to the static condition. n = 3 for 5 dynes and n = 8 for 12 and 20 dynes. Error bars represent the SEM. *, P < 0.05 versus static condition. (B) Western blot of total cell lysates of HUAECs kept in static conditions or exposed to LSS of 20 dynes/cm2 for 1 h for MSX1, pSMAD1/5/8, SMAD1, and GAPDH. (C) IF staining on HUAECs with and without exposure to LSS of 20 dynes/cm2 for 1 h for MSX1 (red), phosphorylated (p)SMAD1/5/8 (green), and Hoechst (nuclear marker in blue) and the corresponding brightfield pictures. The arrows represent the flow direction. Bars, 50 µm.

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