Figure 1. MSX1 is an arterial-specific TF reexpressed during arteriogenic remodeling. (A and B) IF staining for MSX1 or MSX2 (green) and αSMA (red) in unligated (left) and 7-d ligated (right) adductors. DAPI (in blue) was used as a nuclear counterstain with asterisks, arrows, and arrowheads indicating positive ECs, SMCs, and adventitial cells, respectively. (C and D) IF staining for MSX1 or MSX2 (green), αSMA (red), and DAPI (blue) in the right adductor 7 d after femoral artery ligation. (E and F) IF staining for MSX1 or MSX2 (green), isolectin GS-IB4 (red), and DAPI (blue) on sequential sections in the adductor 7 d after femoral artery ligation. (C–F) The white and yellow asterisks indicate positive and negative ECs, respectively. A, artery; V, vein. Bars: (A and C) 25 µm; (B and D) 15 µm; (E and F) 20 µm; (magnifications in C and D) 5 µm; (magnifications in E and F) 20 µm.
Figure 1.

MSX1 is an arterial-specific TF reexpressed during arteriogenic remodeling. (A and B) IF staining for MSX1 or MSX2 (green) and αSMA (red) in unligated (left) and 7-d ligated (right) adductors. DAPI (in blue) was used as a nuclear counterstain with asterisks, arrows, and arrowheads indicating positive ECs, SMCs, and adventitial cells, respectively. (C and D) IF staining for MSX1 or MSX2 (green), αSMA (red), and DAPI (blue) in the right adductor 7 d after femoral artery ligation. (E and F) IF staining for MSX1 or MSX2 (green), isolectin GS-IB4 (red), and DAPI (blue) on sequential sections in the adductor 7 d after femoral artery ligation. (C–F) The white and yellow asterisks indicate positive and negative ECs, respectively. A, artery; V, vein. Bars: (A and C) 25 µm; (B and D) 15 µm; (E and F) 20 µm; (magnifications in C and D) 5 µm; (magnifications in E and F) 20 µm.

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