A dominant mutation in VPS13 suppresses the growth defect of ERMES mutants. (A) Serial dilutions of strains of the indicated genotypes on fermentable (YPD: YP + 2% dextrose) or nonfermentable (YPEG: YP + 3% glycerol + 1.5% ethanol) media. Top: WT. Middle: isogenic mmm1Δ strain from a published deletion library (Giaever et al., 2002) that bears a suppressor mutation (SUP⁺). Bottom: isogenic mmm1Δ strain without suppressor mutation (SUP⁻). (B) Tetrad analysis of an MMM1/mmm1Δ heterozygote (left), a MMM1/mmm1Δ; SUP⁻/SUP⁺ heterozygote (middle), and a mmm1Δ homozygote, SUP⁻/SUP⁺ heterozygote (right). (C) Quality scores of SNPs were classified in three categories: SNPs found into both the SUP⁺ and SUP⁻ DNA pools (left), SNPs found in the SUP⁻ pool only (middle), and SNPs found in the SUP⁺ pool (right). Low-scoring SNPs represent sequencing errors while high scoring ones represent bona fide variants. (D) A diploid MMM1/mmm1Δ heterozygote was transformed with a plasmid encoding WT Vps13 (pVPS13, left) or the D716H allele (pVPS13(D716H), right). Spores circled in red bear both the deletion allele and the indicated plasmid. (E) A CEN/ARS plasmid bearing the VPS13(L1627S) allele (pVPS13(L1627S)) or its WT counterpart (pVPS13) were transformed into mmm1Δ and mdm10Δ cells. Transformants were spotted and streaked on YPD.