Figure 5. APPL endosomes exhibit features of cargo sorting by live cell imaging. (A) Sequential images showing an EEA1-positive APPL1-negative vesicle carrying EGF, which fuses with multiple preexisting APPL1 endosomes (see Video 5). (B) Sequential images depicting fusion of a double APPL1+EEA1–positive endosome with an APPL1-positive endosome (see Video 6). (C) Double APPL1+EEA1 endosome gradually loses APPL1 and converts to EEA1 endosome (see Video 7). (D) Double APPL1+EEA1–positive endosome gradually loses EEA1 and converts to APPL1 endosome (see Video 8). (E) Relative frequencies of individual events of interaction between APPL1- (A), EEA1- (E), and double APPL1+EEA1 (AE)–positive endosomes (error bars represent SEMs). Data were collected from 34 movies.Total number of events equals 234.
Figure 5.

APPL endosomes exhibit features of cargo sorting by live cell imaging. (A) Sequential images showing an EEA1-positive APPL1-negative vesicle carrying EGF, which fuses with multiple preexisting APPL1 endosomes (see Video 5). (B) Sequential images depicting fusion of a double APPL1+EEA1–positive endosome with an APPL1-positive endosome (see Video 6). (C) Double APPL1+EEA1 endosome gradually loses APPL1 and converts to EEA1 endosome (see Video 7). (D) Double APPL1+EEA1–positive endosome gradually loses EEA1 and converts to APPL1 endosome (see Video 8). (E) Relative frequencies of individual events of interaction between APPL1- (A), EEA1- (E), and double APPL1+EEA1 (AE)–positive endosomes (error bars represent SEMs). Data were collected from 34 movies.Total number of events equals 234.

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