APPL endosomes are stable structures. (A) Gallery of images showing a long-lived APPL endosome (arrow) containing internalized Tf, tracked for 12 min in HeLa cells expressing EGFP-APPL1 (see Video 1). (B) APPL1 (green) endosome with EGF (blue) and Tf (red) sorts Tf from EGF. Tf-positive tubule growing over time and pinched-off from APPL1 endosome (see Video 3). (C) Double EEA1 (blue) + APPL1 (green) endosome produces Tf- (red) and APPL1-positive, EEA1-negative tubule (see Video 4). Later this tubule was separated from the main endosome body.