![Figure 2. Immunoelectron microscopic localization of APPL1. HeLa cells were labeled with antibodies to APPL1, followed by a Nanogold-labeled conjugate. Gold particles were visualized by silver enhancement. (A) A low-magnification view with two APPL-positive structures circled in orange, one of which is shown at higher magnification in the inset. (B–F) A gallery of representative structures. Dense labeling is associated with small tubular profiles close to the PM or deeper inside the cell as well as with larger heterogeneous structures. Note that labeling of the PM or CCPs is very low. M, mitochondria. Bars: (A, C, and E) 500 nm; (A [inset], B, D, and F) 200 nm.](https://cdn.rupress.org/rup/content_public/journal/jcb/211/1/10.1083_jcb.201311117/6/jcb_201311117_fig2.jpeg?Expires=1767645793&Signature=x5pyl0vRFv2EvYXQ5Zj9pCl1567YHcvK0J7MlMav9qeM6d24636-u~dKjtQPkxpb82y7MTR7Rv0bG9IwmvXcUOi5v4-YFLhlNQZ5NuqT-UwWbEcxH9uMU1dx5zJSuIe~KMGIobP6Tax3mUny2MxSqssntv7eIvoKwn1dh~5oBaJ2z0URRwpL5KDDVJJ8CN0Ciy2wEmXHak7AlsIH~vpso~FycMWeUELyBd6Rs~lzy1boYk6UDPyNNK3F3pcQ6-zdc79N7LxJKrhqg46rj2EbtLlaiHkRSQiz6KnPdUzEyb0vIuywbqmEiP8MVoA0rklvtA4FjZYiUoMt2dKqi3A-Ug__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 2.
Immunoelectron microscopic localization of APPL1. HeLa cells were labeled with antibodies to APPL1, followed by a Nanogold-labeled conjugate. Gold particles were visualized by silver enhancement. (A) A low-magnification view with two APPL-positive structures circled in orange, one of which is shown at higher magnification in the inset. (B–F) A gallery of representative structures. Dense labeling is associated with small tubular profiles close to the PM or deeper inside the cell as well as with larger heterogeneous structures. Note that labeling of the PM or CCPs is very low. M, mitochondria. Bars: (A, C, and E) 500 nm; (A [inset], B, D, and F) 200 nm.
