PI3K–PDK1, but not FAK, act downstream of Src. MCF-10A cells expressing doxycycline-inducible CA-Src-GFP fusion protein were treated with 1 µg/ml doxycycline (Dox) in complete medium for 12 h and starved for 24 h in starvation medium containing doxycycline. Indicated inhibitors were added to culture medium and incubated for 30 min before fixation. Subcellular localization of YAP was determined by immunofluorescence staining. One of three independent results is presented. Tet, tetracycline (or doxycycline) inducible. Bar, 25 µm.