Figure 9. Antagomir-9-3p restores AZD8055-suppressed growth and survival of tumor xenografts. (A and B) BALB/c nude mice were treated with captisol or AZD8055 by gavage and intratumorally injected with antagomirs of NC and miR-9-3p. Each treatment group comprised eight mice. (A) Representative images of mice (top) and MDA-MB-231 xenografts (bottom). (B) Tumor growth curves (left) and tumor weights (right) of MDA-MB-231 xenografts. (C and D) MDA-MB-231 xenografts treated as described in A were either analyzed for the indicated proteins by Western blotting (C) or subjected to TUNEL labeling (D). Images of DAB/hematoxylin staining under a light microscope (40×) are shown. Bar, 50 µm. Arrowheads indicate apoptotic cells. (E) A schematic diagram illustrates the currently defined mTORC2–CIP2A–PP2A–c-Myc–miR-9-3p–E2F1 pathway. Error bars represent mean values ± SEM. NC, negative control.
Figure 9.

Antagomir-9-3p restores AZD8055-suppressed growth and survival of tumor xenografts. (A and B) BALB/c nude mice were treated with captisol or AZD8055 by gavage and intratumorally injected with antagomirs of NC and miR-9-3p. Each treatment group comprised eight mice. (A) Representative images of mice (top) and MDA-MB-231 xenografts (bottom). (B) Tumor growth curves (left) and tumor weights (right) of MDA-MB-231 xenografts. (C and D) MDA-MB-231 xenografts treated as described in A were either analyzed for the indicated proteins by Western blotting (C) or subjected to TUNEL labeling (D). Images of DAB/hematoxylin staining under a light microscope (40×) are shown. Bar, 50 µm. Arrowheads indicate apoptotic cells. (E) A schematic diagram illustrates the currently defined mTORC2–CIP2A–PP2A–c-Myc–miR-9-3p–E2F1 pathway. Error bars represent mean values ± SEM. NC, negative control.

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