Figure 3.
Figure 3. Increased ciliary forces disorient BBs in disA-1 cells. (A) Elevated temperature in cycling cells increases the disA-1 phenotype. BB, red; KF, green. Bars: (left panels) 10 µm; (enlarged panels) 750 nm. (B) Elevated temperature lengthens KFs. n > 280 KFs. (C) Elevated temperature increases disA-1 BB disorientation. n > 54. (D) Elevated temperature increases cell motility. The node spacing represents the distance traveled in 170 ms. Bar, 100 µm. (E) disA-1 cells do not maintain temperature-induced increases in motility. n > 48. (F and G) High-viscosity media lengthens WT KFs (n > 153 KFs) and disA-1 BB disorientation (n > 30) in cycling and G1-arrested cells grown in PEO at 25°C. Brackets indicate the samples being compared, and asterisks indicate statistical significance (P < 0.01). Error bars indicate SEM.

Increased ciliary forces disorient BBs in disA-1 cells. (A) Elevated temperature in cycling cells increases the disA-1 phenotype. BB, red; KF, green. Bars: (left panels) 10 µm; (enlarged panels) 750 nm. (B) Elevated temperature lengthens KFs. n > 280 KFs. (C) Elevated temperature increases disA-1 BB disorientation. n > 54. (D) Elevated temperature increases cell motility. The node spacing represents the distance traveled in 170 ms. Bar, 100 µm. (E) disA-1 cells do not maintain temperature-induced increases in motility. n > 48. (F and G) High-viscosity media lengthens WT KFs (n > 153 KFs) and disA-1 BB disorientation (n > 30) in cycling and G1-arrested cells grown in PEO at 25°C. Brackets indicate the samples being compared, and asterisks indicate statistical significance (P < 0.01). Error bars indicate SEM.

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