Exo70M26p loses actin-independent polarization. (A) Yeast cells expressing WT Exo70p-GFP (NY3052) or various mutants (NY3054, NY3056, NY3057, NY3058, NY3059, and NY3060) were grown to early log phase in synthetic medium at 25°C. Cells were harvested and treated with DMSO or 200 µM LatA for 10 min and then examined by confocal fluorescence microscopy. Bars, 5 µm. (B) The percentage of cells with polarized GFP signal in small-budded or medium/large-budded cells was quantified. Error bars indicate SD for three separate experiments. For each experiment, 200–300 cells were scored for each strain. *, P < 0.001; **, P < 0.002; ***, P < 0.005; Student’s t test.