Figure 1. Exo70p shows localization defects in bem1Δ cells. (A) Yeast cells expressing Exo70p-GFP in WT (NY3052) and a bem1Δ mutant (NY3053) were grown to early log phase in SC-URA medium at 25°C. Cells were harvested and treated with DMSO or 200 µM LatA for 10 min and then examined by confocal fluorescence microscopy. Bars, 5 µm. (B) The percentage of cells with polarized GFP signal in unbudded, small-budded, or medium/large-budded cells was quantified. Error bars indicate SD for three separate experiments. For each experiment, 200–300 cells were scored for each strain. *, P < 0.001; **, P < 0.01; Student’s t test.
Figure 1.

Exo70p shows localization defects in bem1Δ cells. (A) Yeast cells expressing Exo70p-GFP in WT (NY3052) and a bem1Δ mutant (NY3053) were grown to early log phase in SC-URA medium at 25°C. Cells were harvested and treated with DMSO or 200 µM LatA for 10 min and then examined by confocal fluorescence microscopy. Bars, 5 µm. (B) The percentage of cells with polarized GFP signal in unbudded, small-budded, or medium/large-budded cells was quantified. Error bars indicate SD for three separate experiments. For each experiment, 200–300 cells were scored for each strain. *, P < 0.001; **, P < 0.01; Student’s t test.

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