Analyses of the Drosophila mitochondrial acetylome and dSirt2 acetylome reveal extensive acetylation of proteins engaged in OXPHOS and metabolic pathways involved in energy production. (A) GO analysis (cellular component) of the acetylome shows significant enrichment of mitochondria-related terms. (B) Distribution of acetyl-Lys sites identified per protein in the mitochondrial acetylome. (C) Pathway analysis of the mitochondrial acetylome with the number of proteins identified per pathway indicated. (D) Consensus sequence logo plot for acetylation sites, ±6 amino acids from all acetyl-Lys identified in the mitochondrial acetylome. (E) GO analysis (cellular component) of the acetylated proteins that increase in the dsirt2 mutant. (F) Pathway analysis of the acetylated proteins that increase in dsirt2 with the number of proteins identified per pathway indicated. (G) Consensus sequence logo plot for acetylation sites, ±6 amino acids from all acetyl-Lys identified in proteins that increase in dsirt2.