Deletion analysis of the UNC-84 luminal domain. (A) Schematics of the UNC-84 luminal domain deletions are presented along with the mean number of dorsal cord nuclei observed in L1 larva. Data for one representative line are shown for all deletions except unc-84(Δ861–894) and unc-84(Δ915–919), both of which displayed varying means in three independent lines. Error bars denote standard deviations. (B–D) Transgenic unc-84(n369) mutant embryos expressing UNC-84 luminal domain deletion constructs were stained with anti–UNC-84 (left) and anti–UNC-83 (right) antibodies. unc-84(Δ556–861) retained the ability to recruit UNC-83 to the NE (D; arrows). For all images, anterior is left, and dorsal is up. Bar, 10 µm.