Figure 9.
Figure 9. HDAC6 inhibition rescues 3D motility in cells lacking paxillin and is dependent on Golgi cohesion. (A) Western blot of paxillin and GM130 RNAi in MDA-MB-231 cells. (B) Images of MDA-MB-231 cells treated with GM130 RNAi. (C and D) Quantitation of migration velocity (C) and directionality of cells (D) treated with RNAi as indicated migrating through 3D CDMs. n = minimum of 25 cell tracks from three individual experiments. Data are represented as the mean ± SEM. **, P < 0.005. (E) Immunofluorescence images of the Golgi indicating that, in the absence of GM130, tubacin is unable to rescue the Golgi fragmentation or elongated cell morphology observed in cells lacking paxillin in 3D CDM. Boxed regions indicate the area used for the respective zoomed giantin inset images.

HDAC6 inhibition rescues 3D motility in cells lacking paxillin and is dependent on Golgi cohesion. (A) Western blot of paxillin and GM130 RNAi in MDA-MB-231 cells. (B) Images of MDA-MB-231 cells treated with GM130 RNAi. (C and D) Quantitation of migration velocity (C) and directionality of cells (D) treated with RNAi as indicated migrating through 3D CDMs. n = minimum of 25 cell tracks from three individual experiments. Data are represented as the mean ± SEM. **, P < 0.005. (E) Immunofluorescence images of the Golgi indicating that, in the absence of GM130, tubacin is unable to rescue the Golgi fragmentation or elongated cell morphology observed in cells lacking paxillin in 3D CDM. Boxed regions indicate the area used for the respective zoomed giantin inset images.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal