Figure 8. Expression of the nonacetylatable α-tubulinK40R mutant is sufficient to promote Golgi fragmentation and a nonmotile paxillin RNAi-like 3D morphology. (A and B) Images (A) and quantitation (B) of Golgi object number per cell in parental MDA-MB-231 cells expressing GFP-tagged constructs as indicated. Boxed regions indicate the area used for the respective zoomed giantin inset images. (C) Images to indicate that expression of either GFP-tagged α-tubulin construct has no gross effect on the overall MT network. (D) Representative images of MDA-MB-231 cells migrating through 3D CDMs indicating that, in contrast to the wild-type protein, GFP–α-tubulinK40R expression is sufficient to promote a nonmotile hyperelongated morphology. Data are from n = 3 individual repeats and represented as the mean ± SEM. **, P < 0.005; ***, P < 0.0005.
Figure 8.

Expression of the nonacetylatable α-tubulinK40R mutant is sufficient to promote Golgi fragmentation and a nonmotile paxillin RNAi-like 3D morphology. (A and B) Images (A) and quantitation (B) of Golgi object number per cell in parental MDA-MB-231 cells expressing GFP-tagged constructs as indicated. Boxed regions indicate the area used for the respective zoomed giantin inset images. (C) Images to indicate that expression of either GFP-tagged α-tubulin construct has no gross effect on the overall MT network. (D) Representative images of MDA-MB-231 cells migrating through 3D CDMs indicating that, in contrast to the wild-type protein, GFP–α-tubulinK40R expression is sufficient to promote a nonmotile hyperelongated morphology. Data are from n = 3 individual repeats and represented as the mean ± SEM. **, P < 0.005; ***, P < 0.0005.

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