Reexpression of a paxillin mutant lacking the proline-rich region is unable to rescue Golgi fragmentation and a motile phenotype. (A and B) Images of MDA-MB-231 cells treated with paxillin RNAi, reexpressing GFP-tagged paxillin constructs as indicated spread on 2D fibronectin (FN; A) and 3D CDMs (B). The average number of Golgi objects/cell and statistical significance relative to the GFP control are indicated. n = minimum of 10 cells.