Figure 6. Paxillin depletion in MDA-MB-231 cells promotes Golgi fragmentation in 2D and 3D microenvironments, which can be rescued by HDAC6 inhibition. (A) Images of the Golgi fragmentation phenotype observed in MDA-MB-231 cells lacking paxillin. Boxed regions indicate the area used for the respective zoomed giantin images. (B–D) Quantitation of the percentage of RNAi-treated cells with a fragmented Golgi (B) and the number (C) and average area (D) of discrete Golgi objects per cell. (E) Western blots indicating no significant change in expression of the Golgi protein giantin in cells treated with paxillin RNAi. (F–H) Images (F) and quantitation of Golgi object number (G) and area in cells treated with RNAi (H) as stated. Where applicable, cells were treated with 2 µM tubacin 4 h before fixation. Data are represented as the mean ± SEM. **, P < 0.005; ***, P < 0.0005.
Figure 6.

Paxillin depletion in MDA-MB-231 cells promotes Golgi fragmentation in 2D and 3D microenvironments, which can be rescued by HDAC6 inhibition. (A) Images of the Golgi fragmentation phenotype observed in MDA-MB-231 cells lacking paxillin. Boxed regions indicate the area used for the respective zoomed giantin images. (B–D) Quantitation of the percentage of RNAi-treated cells with a fragmented Golgi (B) and the number (C) and average area (D) of discrete Golgi objects per cell. (E) Western blots indicating no significant change in expression of the Golgi protein giantin in cells treated with paxillin RNAi. (F–H) Images (F) and quantitation of Golgi object number (G) and area in cells treated with RNAi (H) as stated. Where applicable, cells were treated with 2 µM tubacin 4 h before fixation. Data are represented as the mean ± SEM. **, P < 0.005; ***, P < 0.0005.

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