Figure 3.
Figure 3. Map1 is adjacent to NAC at the UAS. (A) Ribosome fractions derived from WT (BY4741) and Δnac strains were treated with the cross-linker GMBS (200 µM) and analyzed by Western blot for Map1. (B) Ribosome fractions from WT yeast (W303) with or without the pTDH-MAP1 plasmid (pMP299), as well a Δmap1 (GFY9) strain complemented with a Map1FLAG plasmid (pMP289), were treated with GMBS as in A and analyzed by Western blot for Egd2. (C) Cross-linking of ribosome fractions from WT (RPL35, W303) and a Δrpl35A/B strain expressing a C-terminally HA-tagged RPL35A (RBY175) were performed with DSS followed by Western blot for Egd2. (D) Ribosome pellets from indicated strains were analyzed by Western blot for Egd2, Rps3, and Rpl35.

Map1 is adjacent to NAC at the UAS. (A) Ribosome fractions derived from WT (BY4741) and Δnac strains were treated with the cross-linker GMBS (200 µM) and analyzed by Western blot for Map1. (B) Ribosome fractions from WT yeast (W303) with or without the pTDH-MAP1 plasmid (pMP299), as well a Δmap1 (GFY9) strain complemented with a Map1FLAG plasmid (pMP289), were treated with GMBS as in A and analyzed by Western blot for Egd2. (C) Cross-linking of ribosome fractions from WT (RPL35, W303) and a Δrpl35A/B strain expressing a C-terminally HA-tagged RPL35A (RBY175) were performed with DSS followed by Western blot for Egd2. (D) Ribosome pellets from indicated strains were analyzed by Western blot for Egd2, Rps3, and Rpl35.

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