Rpgrip1l localizes at the ciliary TZ in G₀ phase and at centrosomes during mitosis. (A–C) Immunofluorescence on MEFs (isolated from E12.5 embryos). (A and B) Plots indicate fluorescent intensities of each channel along the cilium from base (left) to tip (right). (A) The ciliary axoneme is marked by acetylated α-tubulin (α-Tub) and the BB by γ-tubulin. (B) The ciliary axoneme is marked by acetylated α-tubulin (α-Tub), and the TZ is marked by Cep290. (C) Centrosomes are marked by γ-tubulin, and cell nuclei are marked by DAPI. Insets illustrate higher magnifications of boxed regions. (D) Immunofluorescence on E12.5 murine limbs. The ciliary axoneme is marked by acetylated α-tubulin and centrosomes by Pcnt2. Bars: (A and B) 1 µm; (C) 10 µm; (D, overview) 5 µm; (D, magnification) 1 µm.