Figure 5. The Q-MT bundle rapidly elongates upon quiescence exit. (A) MT cytoskeleton organization before and upon quiescence exit. Quiescent cells (7 d) were refed with YPDA ± CHX or with water containing 2% glucose. The number of MT bundles per cell (top) and the length of the MT bundles (bottom) were then measured (N = 2 experiments and n > 200). Representative cells expressing GFP-Atb2 are shown. Means and SDs are indicated. (B) Time-lapse movies of cell coexpressing RFP-Atb2 and Alp14-GFP upon quiescence exit. Alp14 dots (pink arrowhead) follow MT growing tips. Quiescent cells (5 d) were refed with YPDA. The time after cell refeeding is indicated in minutes. (C) The Q-MT bundle stays stable upon quiescence exit. Quiescent cells (7 d) were incubated 30 min in YPDA ± CHX ± TBZ. MT bundle length (top) and fluorescence intensity (bottom) were measured; p-values are indicated. Representative cells are shown. Bars: (A and C) 2 µm; (B) 0.5 µm.
Figure 5.

The Q-MT bundle rapidly elongates upon quiescence exit. (A) MT cytoskeleton organization before and upon quiescence exit. Quiescent cells (7 d) were refed with YPDA ± CHX or with water containing 2% glucose. The number of MT bundles per cell (top) and the length of the MT bundles (bottom) were then measured (N = 2 experiments and n > 200). Representative cells expressing GFP-Atb2 are shown. Means and SDs are indicated. (B) Time-lapse movies of cell coexpressing RFP-Atb2 and Alp14-GFP upon quiescence exit. Alp14 dots (pink arrowhead) follow MT growing tips. Quiescent cells (5 d) were refed with YPDA. The time after cell refeeding is indicated in minutes. (C) The Q-MT bundle stays stable upon quiescence exit. Quiescent cells (7 d) were incubated 30 min in YPDA ± CHX ± TBZ. MT bundle length (top) and fluorescence intensity (bottom) were measured; p-values are indicated. Representative cells are shown. Bars: (A and C) 2 µm; (B) 0.5 µm.

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