Figure 2. The Q-MT bundle is composed of stable MTs. (A) Q-MT bundles do not display detectable length variation. Variation of MT bundle length as a function of time in interphase and quiescent cells. Representative time-lapse movies are shown. Red and blue arrows point, respectively, at shrinking and growing MTs. (B) Q-MT bundles are insensitive to treatments that destabilize dynamic MTs. The graph displays MT bundle length measured in interphase and in quiescent cells (4 d) incubated 30 min with the indicated drugs. Representative cells are shown. (C) FRAP of GFP-Atb2 within a Q-MT bundle measured in quiescent cells (4 d). Error bars are SEM. Time is in seconds. The red box indicates the photobleached area. In all panels, cells are WT cells expressing GFP-Atb2, Sfi1-CFP, and Cut11-RFP. In A and C, only the GFP channel is shown, and in B GFP-Atb2 is in green and Cut11-RFP is in red. Bars, 2 µm.
Figure 2.

The Q-MT bundle is composed of stable MTs. (A) Q-MT bundles do not display detectable length variation. Variation of MT bundle length as a function of time in interphase and quiescent cells. Representative time-lapse movies are shown. Red and blue arrows point, respectively, at shrinking and growing MTs. (B) Q-MT bundles are insensitive to treatments that destabilize dynamic MTs. The graph displays MT bundle length measured in interphase and in quiescent cells (4 d) incubated 30 min with the indicated drugs. Representative cells are shown. (C) FRAP of GFP-Atb2 within a Q-MT bundle measured in quiescent cells (4 d). Error bars are SEM. Time is in seconds. The red box indicates the photobleached area. In all panels, cells are WT cells expressing GFP-Atb2, Sfi1-CFP, and Cut11-RFP. In A and C, only the GFP channel is shown, and in B GFP-Atb2 is in green and Cut11-RFP is in red. Bars, 2 µm.

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