Kip3 is required to scale anaphase spindle length with cell length. (a) Spindle length was scaled with cell size in late anaphase (cdc15-2, 2 h at 37°C). The graph represents the data from two independent cultures, n = 87. (b) Representative straight (top) and fishhook (bottom) spindle images from cells expressing GFP-tubulin. (c) Schematic (top) and representative image (bottom) of the lasso spindle morphology. (d) Percentage of total anaphase spindles with lasso morphology in control and mutant cells during anaphase arrest (cdc15-2, 2.5 h at 37°C). The graph shows the average ± SE of three separate experiments. P < 0.005 for kip3Δ versus each cell type. For each cell type the compiled n = 112–540, and in each experiment n = 24–271. Bar, 2 µm.