AnkG organization in the AIS coat. Immunogold PREM of AnkG in DIV14 neurons. (A–D) Regions of the AIS coat along the main axon. Gold particles (12 nm; yellow) are present in small clusters or individually in dense regions (A). In stretched or sparse regions of the coat, AnkG immunogold associates with fibrillar networks (B) or globular clusters (C and D). (E) A gallery of individual AnkG-positive structures in the sparse or stretched AIS coat. Duplicate images are pseudocolored to show gold particles (yellow) associated with putative AnkG structures (red), such as long, thin, wavy filaments (e.g., top row) or elongated globular clusters of variable thickness and morphology. A donut-shaped putative ion channel (see Fig. 10) within an AnkG globular cluster is pseudocolored in blue. Bars: (A–D) 100 nm; (E) 20 nm.