PP2A^Rts1 is required for normal control of CLN3 mRNA and protein levels in cells released from a metaphase arrest. (A–C) GAL-CDC20, GAL-CDC20 rts1Δ, GAL-CDC20 ace2Δ, and GAL-CDC20 ace2Δ rts1Δ cells were released from a metaphase arrest at 30°C, and samples were analyzed by qRT-PCR (A). Independent samples were probed for Cln3-6×HA (B) and Clb2 (C) by Western blotting. The Cln3-6×HA and Clb2 Western blots were from the same samples to allow direct comparison of the timing of cell cycle events. (D) Cell growth rate was analyzed using a Bioscreen C apparatus. (E) A series of 10-fold dilutions of cells were grown at 25°C on YEP media containing either dextrose or galactose. Error bars in A indicate the SDs of three biological replicates. Error bars in D indicate SDs of four independent cultures.