PP2A^Rts1 is required for normal control of CLN3 mRNA and protein levels in cells released from a G1 arrest. (A–D) Wild-type, rts1Δ, ace2Δ, and rts1Δ ace2Δ cells were released from G1 arrest at 30°C, and the behavior of CLN3 mRNA was assayed by Northern blotting (A) or qRT-PCR (B). Independent samples were probed for Cln3-6×HA (C) and Clb2 (D) by Western blotting. The Cln3-6×HA and Clb2 Western blots were from the same samples to allow direct comparison of the timing of cell cycle events. Loading controls for the Northern blot and the Western blots are shown in Fig. S2. Error bars in B indicate the SDs of three biological replicates.