The Ace2 transcription factor is hyperphosphorylated in rts1Δ cells. (A) Cells were released from a G1 arrest, and the behavior of Ace2 and Clb2 was assayed by Western blotting. (B) The same samples shown in Fig. 2 B were loaded in an intercalated manner to visualize differences between wild-type and rts1Δ cells. (C) Cells were released from a metaphase arrest, and the behavior of Ace2 and Clb2 was assayed by Western blotting.