NPC inheritance does not depend on de novo NPC assembly but is required for daughter viability. (A) Deconvolved image of a middle z plane showing Nup192-FRB-GFP (PCCPL528) or Nup120-FRB-GFP (WZCPL5) after completion of anaphase in the presence of rapamycin. M and D, mother and daughter, respectively. Bars, 2 µm. (B) Plot of tfd/tfm for Nup192-FRB-GFP and Nup120-FRB-GFP as in Fig. 3. 42 ≤ n ≤ 55. (C) Single mother (M) cells expressing Nsp1-FRB-GFP (PCCPL487) or Nup192-FRB-GFP (PCCPL552) were grown in a microfluidic chamber perfused with rapamycin for 24 h. Each daughter was assigned a number (starting with 1) and their daughters assigned the same number followed by a letter to denote their parentage and order of appearance (starting with “a”). Bars, 5 µm. (D) Quantification of C where the average number of buds from mother and daughter cells is plotted. 29 and 32 mother cells (from two independent experiments) were analyzed for PCCPL487 and PCCPL552 strains, respectively. Error bars indicate standard deviation from the mean.
