Nascent MRs shed numerous CR components, except F-actin. (A and B) Fixed cells expressing Anillin-GFP and tubulin-mCh stained for anti-Pnut (A) and anti-Rho1 (B) by immunofluorescence. Separated channels of boxed regions are shown magnified on the right. (C) Cell expressing Anillin-GFP and Sticky (Sti)-mCh. (D) Cell expressing Anillin-mCh and Tum-GFP. (E) Cell expressing Anillin-mCh and Pavarotti (Pav)-GFP. (F) Inverted look-up table of the nascent MR of an Aurora B–GFP-expressing cell. (G) The nascent MR of a myosin-GFP–expressing cell. Dashed lines mark the cell boundary. (H) The nascent MR of a cell expressing myosin-GFP (Myo) and Anillin-mCh (Ani). (I) The nascent MR of a cell expressing LifeAct-GFP (LA) and Anillin-mCh. (C, D, E, H, and I) Separated channels of boxed regions are shown magnified at the bottom. (J) The nascent MR of a cell expressing Anillin-GFP fixed and stained with rhodamine-phalloidin. Arrowhead points to an extrusion enriched in Anillin-GFP but lacking actin. Times are hours, minutes, and seconds. Bars: (A [main image] and B–J) 5 µm; (A, magnified images) 1 µm. See also Video 3.