Figure 1. ER into LD segregation of... | Rockefeller University Press

Figure 1.

$Figure 1. ER into LD segregation of HPos. (A) Model peptides used in this work. Bold letters indicate the three positive residues that were mutated to glycines. (B) Starved GFP-HPos– or GFP-HNeu–transfected cells (24 h stv) and cells additionally incubated 24 h with OA (24 h OA) were fractionated in sucrose density gradients. The distribution of the peptides in the gradients was analyzed by Western blotting with anti-GFP antibodies. Each panel shown represents a separate gradient. The figure also includes representative fractionations in separate gradients of an ER resident protein (Sec61) and a LD protein (Plin2) detected with specific antibodies. (C–F) Distribution of GFP-HNeu and GFP-HPos in starved cells (C and D) or OA-loaded cells (E and F). Bottom panels show high-magnification areas selected from the corresponding panels on top. Arrows in D indicate pre-LDs. Bars: (top) 20 µm; (bottom) 2 µm.$

ER into LD segregation of HPos. (A) Model peptides used in this work. Bold letters indicate the three positive residues that were mutated to glycines. (B) Starved GFP-HPos– or GFP-HNeu–transfected cells (24 h stv) and cells additionally incubated 24 h with OA (24 h OA) were fractionated in sucrose density gradients. The distribution of the peptides in the gradients was analyzed by Western blotting with anti-GFP antibodies. Each panel shown represents a separate gradient. The figure also includes representative fractionations in separate gradients of an ER resident protein (Sec61) and a LD protein (Plin2) detected with specific antibodies. (C–F) Distribution of GFP-HNeu and GFP-HPos in starved cells (C and D) or OA-loaded cells (E and F). Bottom panels show high-magnification areas selected from the corresponding panels on top. Arrows in D indicate pre-LDs. Bars: (top) 20 µm; (bottom) 2 µm.

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