Figure 8. Loss of FOXO1 expression in keratinocytes results in decreased keratinocyte migration and proliferation and increased apoptosis. Paraffin-embedded cross sections from K14.Cre−.FOXO1L/L and K14.Cre+.FOXO1L/L mice were analyzed by immunofluorescence using an anti-uPAR antibody (A), anti-PCNA antibody (E), or anti-8-OHdG antibody (G), and by TUNEL assay (C). Quantification of uPAR-positive migrating keratinocytes (B), TUNEL-positive apoptotic keratinocytes (D), PCNA-positive proliferative keratinocytes (F), and 8-OHdG–positive keratinocytes (H). Bars: (A and G) 100 µm; (C and E) 200 µm. EP, epidermis; CT, connective tissues. White dashed lines demarcate the epidermis from the dermis. Each value is the mean ± SEM for n = 6–9 mice per group. Asterisks indicate significant changes compared with control mice (P < 0.05).
Figure 8.

Loss of FOXO1 expression in keratinocytes results in decreased keratinocyte migration and proliferation and increased apoptosis. Paraffin-embedded cross sections from K14.Cre−.FOXO1L/L and K14.Cre+.FOXO1L/L mice were analyzed by immunofluorescence using an anti-uPAR antibody (A), anti-PCNA antibody (E), or anti-8-OHdG antibody (G), and by TUNEL assay (C). Quantification of uPAR-positive migrating keratinocytes (B), TUNEL-positive apoptotic keratinocytes (D), PCNA-positive proliferative keratinocytes (F), and 8-OHdG–positive keratinocytes (H). Bars: (A and G) 100 µm; (C and E) 200 µm. EP, epidermis; CT, connective tissues. White dashed lines demarcate the epidermis from the dermis. Each value is the mean ± SEM for n = 6–9 mice per group. Asterisks indicate significant changes compared with control mice (P < 0.05).

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