Figure 2. FOXO1 silencing reduces closure of an in vitro scratch wound and keratinocyte migration. NHEK cells were transfected with control siRNA or siRNA specific for FOXO1 or FOXO3 for 48 h. QRT-PCR and Western blot analysis of FOXO1 (A and B) and FOXO3 (C and D) expression. (E) Quantitative analysis of scratch wound closure at the indicated time points in FOXO1/FOXO3 knockdown cells. (F) Quantitative analysis of scratch wound closure at the indicated time points in FOXO1-overexpressed cells. (G) Transwell migration assay of cells transfected with control or FOXO1 siRNA; the migrated cells were photographed and counted. Real-time PCR analysis of TGF-β1 (H), integrin α3 (I), integrin β6 (J), MMP-3 (K), and MMP-9 (L) mRNA levels in FOXO1 knockdown keratinocytes. Data show mean ± SEM of at least three independent measurements per time point. Asterisks indicate significant increase or decrease compared with cells transfected with control siRNA (P < 0.05).
Figure 2.

FOXO1 silencing reduces closure of an in vitro scratch wound and keratinocyte migration. NHEK cells were transfected with control siRNA or siRNA specific for FOXO1 or FOXO3 for 48 h. QRT-PCR and Western blot analysis of FOXO1 (A and B) and FOXO3 (C and D) expression. (E) Quantitative analysis of scratch wound closure at the indicated time points in FOXO1/FOXO3 knockdown cells. (F) Quantitative analysis of scratch wound closure at the indicated time points in FOXO1-overexpressed cells. (G) Transwell migration assay of cells transfected with control or FOXO1 siRNA; the migrated cells were photographed and counted. Real-time PCR analysis of TGF-β1 (H), integrin α3 (I), integrin β6 (J), MMP-3 (K), and MMP-9 (L) mRNA levels in FOXO1 knockdown keratinocytes. Data show mean ± SEM of at least three independent measurements per time point. Asterisks indicate significant increase or decrease compared with cells transfected with control siRNA (P < 0.05).

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