ROCK and MLCK have spatially distinct roles. (a and b) Images (top) and edge velocity map (bottom) of a representative 4-dpf cell before and after treatment with 50 µM ROCKout (a) or 10 µM blebbistatin (b). Arrowheads indicate the time of drug addition. (c) Images of mApple-myosin light chain distribution in a representative 4-dpf cell before and after treatment with ROCKout (top) or ML7 (bottom). Bars, 10 µm. Indicated times are relative to the time of drug addition.