Zebrafish embryonic keratocytes display different behaviors depending on developmental stage. (a) Representative images of cells derived from 2-dpf and 4-dpf embryos. Cell outlines are shown on the right. Bar, 10 µm. (b) Typical edge velocity map for a 2-dpf cell, showing one leading edge. Color represents edge velocity, where red is protruding and blue is retracting (see color scale on the right). (c) Typical edge velocity map for a 4-dpf cell, showing three protrusions in this case, which propagate laterally around the cell. (d) Representative immunofluorescence images of 2-dpf and 4-dpf cells. Cells were fixed and stained with AF594-phalloidin to visualize F-actin, a phospho-serine19 myosin light chain antibody to visualize myosin, and a phospho-tyrosine antibody to visualize adhesions. A maximum intensity projection of a z stack acquired using 3D structured illumination microscopy is shown. Bar, 10 µm.