Figure 4.

Image processing: segmentation and template matching. (a) Automated segmentation of actin filaments in Dictyostelium discoideum: actin filaments (yellow to red); ribosomes (blue). Reproduced with permission from Rigort et al. (2012b); copyright Elsevier, Inc. (b) Polyribosomes from intact human glioma cells identified by template matching. Modified with permission from Brandt et al. (2010); copyright Elsevier, Inc. (c) Protein identification using multiple template matching in intact Leptospira interrogans. Tomographic slices and 3D visualization of identified proteins of nonstimulated (left) and antibiotics-treated cells (right), ribosomes (red), GroEL (light blue), GroEL/ES (dark blue), and RNA polymerase II (green). Bar, 200 nm. Reprinted by permission from Macmillan Publishers Ltd: Nature Methods (Beck et al., 2009); copyright 2009.

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