Figure 5. TYRP1 that accumulates in the Golgi of BLOC-2− melanocytes is not newly synthesized. WT melan-Ink4a or BLOC-2− cells on coverslips were treated with CHX at 37°C for the indicated times, and then fixed, labeled for TYRP1 and the Golgi marker giantin, and analyzed by IFM. (a) Representative deconvolved images of TYRP1 labeling at the 0 and 120 min time points. Red outline, Golgi region defined by giantin labeling. Bar, 10 µm. (b) The intensity of TYRP1 in the giantin-positive Golgi region was quantified relative to total TYRP1 fluorescence and plotted over time. Data represent means ± SD from ≥50 cells in three independent experiments. *, P < 0.05; **, P < 0.01.
Figure 5.

TYRP1 that accumulates in the Golgi of BLOC-2− melanocytes is not newly synthesized. WT melan-Ink4a or BLOC-2− cells on coverslips were treated with CHX at 37°C for the indicated times, and then fixed, labeled for TYRP1 and the Golgi marker giantin, and analyzed by IFM. (a) Representative deconvolved images of TYRP1 labeling at the 0 and 120 min time points. Red outline, Golgi region defined by giantin labeling. Bar, 10 µm. (b) The intensity of TYRP1 in the giantin-positive Golgi region was quantified relative to total TYRP1 fluorescence and plotted over time. Data represent means ± SD from ≥50 cells in three independent experiments. *, P < 0.05; **, P < 0.01.

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