Figure 2. TYRP1 but not TYR is partially mislocalized to early endosomes in the periphery of BLOC-2− cells. (a–o) BLOC-2− (a–c and g–l) and BLOC-2R (d–f and m–o) melanocytes were analyzed by deconvolution IFM after double labeling for TYRP1 (a–i) and STX13 (a–f) or LAMP2 (g–i), or transfection with EGFP-STX13 and labeling for TYR (j–o). Bright-field (BF) images are shown in c, f, i, l, and o. Insets, 3× (j–o) or 3.5× (all others) magnifications of the boxed regions as merged images with bright-field images pseudocolored blue. Arrows in insets, examples of overlap between TYRP1 or TYR and STX13 in BLOC-2− cells; arrowheads, examples of overlap between TYRP1 or TYR and pigment granules; large arrows, perinuclear accumulation of TYRP1 or TYR in BLOC-2− cells. Bars, 10 µm.
Figure 2.

TYRP1 but not TYR is partially mislocalized to early endosomes in the periphery of BLOC-2− cells. (a–o) BLOC-2− (a–c and g–l) and BLOC-2R (d–f and m–o) melanocytes were analyzed by deconvolution IFM after double labeling for TYRP1 (a–i) and STX13 (a–f) or LAMP2 (g–i), or transfection with EGFP-STX13 and labeling for TYR (j–o). Bright-field (BF) images are shown in c, f, i, l, and o. Insets, 3× (j–o) or 3.5× (all others) magnifications of the boxed regions as merged images with bright-field images pseudocolored blue. Arrows in insets, examples of overlap between TYRP1 or TYR and STX13 in BLOC-2− cells; arrowheads, examples of overlap between TYRP1 or TYR and pigment granules; large arrows, perinuclear accumulation of TYRP1 or TYR in BLOC-2− cells. Bars, 10 µm.

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