Mutations in the BUB-1 kinase domain delay anaphase onset to nearly the same extent as BUB-1 depletion. (A) Schematic of RNAi-resistant bub-1 transgene and three transgene-encoded variants. (B) BUB-1 immunoblots of the indicated conditions; α-tubulin is a loading control. (C) Embryo viability analysis for the indicated strains and conditions. Error bars indicate the SD of individual worm progeny viability. (D) Images from representative time-lapse sequences as in Fig. 1 A. Bar, 5 µm. (E) Plot of mean NEBD–anaphase onset intervals for the indicated conditions. Error bars indicate SD; the red broken line is the control value; n is the number of embryos. (F) Plot of the mean delay in anaphase onset for the indicated conditions, generated by subtracting the matched control values and propagating errors. Error bars indicate the 95% CI. bub-1(RNAi) and bub1&mad-2(RNAi) values are from Fig. 1 C; other data are from E. (G) Plot of mean chromosomal GFP fluorescence of the separase sensor, for the indicated conditions. Error bars indicate the 95% CI.