FOCAL POINT Reagan Ching (left), David Bazett-Jones (right), and colleagues (not pictured) describe an unbiased method to identify genomic loci that specifically interact with subnuclear structures like promyelocytic leukemia nuclear bodies (PML NBs). The researchers use an anti-PML antibody to direct the biotinylation of chromatin in close proximity to these mysterious nucleoplasmic particles. By isolating and analyzing the biotinylated chromatin, Ching et al. identify loci that associate with PML NBs in a cell type–specific manner. In situ hybridization can confirm these associations, showing, for example, that the TFF1 gene (red, center panel) is enriched at PML NBs (green), whereas the BCL2 locus (red, right panel) shows no such association.

FOCAL POINT Reagan Ching (left), David Bazett-Jones (right), and colleagues (not pictured) describe an unbiased method to identify genomic loci that specifically interact with subnuclear structures like promyelocytic leukemia nuclear bodies (PML NBs). The researchers use an anti-PML antibody to direct the biotinylation of chromatin in close proximity to these mysterious nucleoplasmic particles. By isolating and analyzing the biotinylated chromatin, Ching et al. identify loci that associate with PML NBs in a cell type–specific manner. In situ hybridization can confirm these associations, showing, for example, that the TFF1 gene (red, center panel) is enriched at PML NBs (green), whereas the BCL2 locus (red, right panel) shows no such association.

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