Figure 1. Ptc restricts ISC proliferation in Drosophila adult midgut. (A–K) 3- to 5-d-old adult female flies were heat shocked at 37°C for 1 h to induce MACRM clones for control FRT chromosomes (A, A′, F, F′, I, and I′), FRT42 ptcIIW (B and B′), FRT42 ptcS2 (C and C′), FRT42 cos22 (D and D′), or FRT40 smo3 chromosome (G, G′, J, and J′) and cultured at 18°C for 10 (A–H) or 20 d ACI (I–K). Midguts were immunostained for GFP (green) and DRAQ5 (blue) and clone size was quantified for each genotype (E, H, and K). Clones were marked by GFP and nuclei were marked by DRAQ5. Only ISC lineage clones in the posterior region of midguts were included for quantification. Bars, 50 µm. Data are mean ± SEM. n = 150. ***, P < 0.001. ns, not significant.
Figure 1.

Ptc restricts ISC proliferation in Drosophila adult midgut. (A–K) 3- to 5-d-old adult female flies were heat shocked at 37°C for 1 h to induce MACRM clones for control FRT chromosomes (A, A′, F, F′, I, and I′), FRT42 ptcIIW (B and B′), FRT42 ptcS2 (C and C′), FRT42 cos22 (D and D′), or FRT40 smo3 chromosome (G, G′, J, and J′) and cultured at 18°C for 10 (A–H) or 20 d ACI (I–K). Midguts were immunostained for GFP (green) and DRAQ5 (blue) and clone size was quantified for each genotype (E, H, and K). Clones were marked by GFP and nuclei were marked by DRAQ5. Only ISC lineage clones in the posterior region of midguts were included for quantification. Bars, 50 µm. Data are mean ± SEM. n = 150. ***, P < 0.001. ns, not significant.

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