RAD51 promotes DNA2-depedent degradation of reversed replication forks. (A) Representative IdU tracts in RECQ1-, RAD51-, or RECQ1/RAD51-codepleted U-2 OS cells (out of 2 repeats). Above, RECQ1 and RAD51 expression after siRNA knockdown RAD51-WT are U-2 OS cells stably expressing siRNA resistant exogenous RAD51. (B) Representative IdU tracts in U-2 OS cells expressing exogenous RAD51 (out of 2 repeats). n ≥ 300 tracts scored for each dataset shown in A and B.