Figure 7. Kindlin2 is required for invadopodia-mediated degradation of dense collagen. (A) Depletion of kindlin2 using kindlin2-specific single duplex siRNA inhibits degradation of HDFC collagen visualized by immunofluorescence of 1/4 collagen type I fragments. (B) Quantification of effect of kindlin2 depletion by single duplex siRNA on matrix degradation in A. Values are means ± SEM of ∼20 cells. AU, arbitrary unit. (C) Effect of kindlin2 knockdown on invadopodia formation in HT-1080 breast adenocarcinoma and PC3 prostate carcinoma cells. Values are means ± SEM of ∼100 cells/condition with three repeats. (D) Representative images after kindlin2 depletion in HT-1080 and PC3 cells. N/S, nonspecific; N/T, not transfected. *, P < 0.05; **, P < 0.001; ***, P < 0.0001. Bars, 10 µm.
Figure 7.

Kindlin2 is required for invadopodia-mediated degradation of dense collagen. (A) Depletion of kindlin2 using kindlin2-specific single duplex siRNA inhibits degradation of HDFC collagen visualized by immunofluorescence of 1/4 collagen type I fragments. (B) Quantification of effect of kindlin2 depletion by single duplex siRNA on matrix degradation in A. Values are means ± SEM of ∼20 cells. AU, arbitrary unit. (C) Effect of kindlin2 knockdown on invadopodia formation in HT-1080 breast adenocarcinoma and PC3 prostate carcinoma cells. Values are means ± SEM of ∼100 cells/condition with three repeats. (D) Representative images after kindlin2 depletion in HT-1080 and PC3 cells. N/S, nonspecific; N/T, not transfected. *, P < 0.05; **, P < 0.001; ***, P < 0.0001. Bars, 10 µm.

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