Proposed model for the phosphoregulation of EB3 function during mitotic exit and cytokinesis. In late mitosis, an Aurora B phosphorylation gradient ensures that only MTs in the furrow region remain phosphorylated, whereas MTs in the vicinity of the substrate contain dephosphorylated EB3. Phosphorylation of EB3 at S176 by Aurora B ensures successful cytokinesis completion by promoting midbody MT stability and midbody stabilization. Dephosphorylation of EB3 occurs near the substrate and restricts MT growth, allowing for coordinated daughter cell spreading, which, by itself, indirectly potentiates the successful completion of cytokinesis.