Characterization of postmitotic cell adhesion to the substrate. (A) HeLa cells were filmed using phase-contrast microscopy. 0 min corresponds to the first frame in anaphase. Arrows indicate cells with uncoordinated adhesion. Arrowheads and insets indicate cells expressing either the full-length EB3-GFP (EB3-FL) or the MT binding domain of EB3 (EB3-MT) that were tracked in this particular example. Bars, 10 µm. (B) Quantification of the adhesion delay between the first and the second daughter cells (*, P < 0.001 using nonparametric ANOVA followed by a post-hoc Dunn’s test). Experiments were done in triplicate and N represents the number of cells quantified in each condition.