Equal segregation of Notch-containing endosomes in dividing SOPs. (A–B‴) Snapshots of a three-color video (GFP, Cherry, and nlsFP670, blue) showing the distribution of NiGFP4Cherry5 in mitotic SOPs (marked by nlsFP670) at two time points (t = 0, onset of cytokinesis, in A–A‴; t = 3 min in B–B‴). Top (apical) and bottom (basal) images show two confocal sections. Cherry-positive endosomes are shown with boxes (note that some endosomes were best detected at focal planes other than the one shown in A–B‴). Bar, 5 µm. (C) Box plot showing the number of Cherry-positive endosomes in pIIb and pIIa at t = 0 and t = 3 (n = 12; n is the number of SOPs; in this and other box plots, outliers are shown as small circles). No significant difference was observed in the number of Cherry-positive between pIIa and pIIb at t = 0 and t = 3 or between t = 0 and t = 3 in pIIb and pIIa. Box and whisker plots are uniform in their use of the box: the bottom and top of the box are the first and third quartiles, and the band inside the box is the second quartile (the median). The ends of the whiskers represent the lowest datum still within 1.5 interquartile range (IQR) of the lower quartile and the highest datum still within 1.5 IQR of the upper quartile (often called the Tukey box plot).