Figure 2. Maturation of Cherry-tagged Notch. (A and A′) Cherry fluorescence of NiCherry (red) was detected at the apical plasma membrane in krz mutant cells (marked by the loss of the GFP marker; clone border indicated in white) but not in wild-type cells. This indicated that slowing down the turnover of Notch at the cell surface by removing the activity of krz allowed for the maturation of Cherry. Bar, 5 µm. (B) Diagram illustrating the time-dependent maturation of NiGFP4Cherry5. Initially, dark NiGFP4Cherry5 (white spots) is synthesized. Because GFP is maturing faster than Cherry, bright NiGFP4Cherry5 mostly include GFP-positive (green spot) molecules when turnover is rapid (wild-type cells, left), whereas some GFP/Cherry-positive molecules (green and red spots) could be detected when turnover is slow (krz mutant cells, right). The horizontal purple arrow indicates time.
Figure 2.

Maturation of Cherry-tagged Notch. (A and A′) Cherry fluorescence of NiCherry (red) was detected at the apical plasma membrane in krz mutant cells (marked by the loss of the GFP marker; clone border indicated in white) but not in wild-type cells. This indicated that slowing down the turnover of Notch at the cell surface by removing the activity of krz allowed for the maturation of Cherry. Bar, 5 µm. (B) Diagram illustrating the time-dependent maturation of NiGFP4Cherry5. Initially, dark NiGFP4Cherry5 (white spots) is synthesized. Because GFP is maturing faster than Cherry, bright NiGFP4Cherry5 mostly include GFP-positive (green spot) molecules when turnover is rapid (wild-type cells, left), whereas some GFP/Cherry-positive molecules (green and red spots) could be detected when turnover is slow (krz mutant cells, right). The horizontal purple arrow indicates time.

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