Multipolar LIC1 and 2 morphant spindle poles contain single centrioles. (A–C) Spindle pole composition was analyzed in control (Ctrl; A) and LIC1 and 2 morphant (B and C) embryos using 3View EM. Chromosomes (blue) and centrioles (red) were reconstructed using Imaris software. Sequential z slices of spindle poles showing a pair of centrioles in control (Ai and Aii) and single centrioles in a multipolar LIC morphant spindle (Bi–Bv). (Ci and Cii) An LIC morphant cell with a bipolar spindle has an increased distance (white double-headed arrows) between centrioles compared with control. Bars: (A, B, and C) 5 µm; (Ai, Aii, Bi–Biv, and Cii) 1 µm. Black arrows mark centrioles.