Mitosis is prolonged when both LICs are depleted. (A) Mitotic index of HeLaMs after KnDs (>731 cells in total per condition, three independent experiments; means ± SEM. ***, P < 0.001, Student’s t test). (B) The duration of mitosis after depleting LICs or DHC or lamin A/C (LA) was determined from phase contrast time-lapse videos. The plot shows the percentage of cells completing mitosis in a given time (158–1,133 cells per treatment combined from three independent experiments; see Results for survival analysis p-values). (C) Mitotic index of epithelial cells in Xenopus embryos injected with a control MO (Ctrl) or MOs targeting LIC 1, 2, or both LICs (*, P < 0.05; ***, P < 0.001; one-way ANOVA, n = 3 independent experiments; means ± SEM). (D) Mitotic progression in Xenopus embryos injected with control or LIC1 and LIC2 MOs was assessed by time-lapse confocal imaging and plotted as a cumulative frequency (n = 43 and 51 spindles analyzed in four and five independent experiments for control MO and LIC1 plus LIC2 MOs, respectively).