SOCE blockade inhibits invadopodium maturation. (A) Live cell imaging showing invadopodium precursor assembly and gelatin degradation by mature invadopodia in control (top) and 100 µM 2-APB–treated (bottom) cells. Arrowheads indicate nascent invadopodia. Bars: (main images) 10 µm; (magnified views) 5 µm. (B and C) Quantification of nascent invadopodium assembly (cortactin-GFP fluorescence intensity) and ECM degradation by nascent invadopodia in control (B) or 2-APB–treated cells (C). Lines in B and C are curve fittings of cortactin-GFP intensity and gelatin degradation index data. The data shown are representative of three experimental repeats. RU, relative unit.